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T25
MA104、MA104、MA104罗猴胎肾细胞 MA104
Cell line name MA-104
Synonyms Ma-104; MA 104; MA104; Microbiological Associates-104
Accession CVCL_3845
Resource Identification Initiative To cite this cell line use: MA-104 (RRID:CVCL_3845)
Comments Group: Non-human primate cell line.
Problematic cell line: Misidentified. Originally thought to be of Rhesus macaque origin but found to be from African Green monkey (PubMed=4043530; PubMed=20143388) and more precisely from Chlorocebus pygerythrus (PubMed=34737324).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00077.
Virology: Susceptible to infection by many viruses (arboviruses, reoviruses, rotaviruses, etc.).
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV). Produces a lytic infection (PubMed=15731278; PubMed=16494729).
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Chlorocebus pygerythrus (Vervet monkey) (Cercopithecus pygerythrus) (NCBI Taxonomy: 60710)
Hierarchy Children:
CVCL_1K12 (9009B) CVCL_A0XU (CL2621) CVCL_3846 (MA-104 Clone 1)
CVCL_4540 (MARC-145) CVCL_3774 (PSP-36)
Sex of cell Sex unspecified
Age at sampling Fetus
Category Spontaneously immortalized cell line
PubMed=4626908; DOI=10.1128/am.24.1.62-69.1972; PMCID=PMC380548
Myers M.G., Vincent M.M., Hensen S.A., Tauraso N.M.
Problems in the laboratory isolation of simian hemorrhagic fever viruses and isolation of the agent responsible for the Sussex/69 epizootic.
Appl. Microbiol. 24:62-69(1972)
PubMed=4043530
Whitaker A.M., Hayward C.J.
The characterization of three monkey kidney cell lines.
Dev. Biol. Stand. 60:125-131(1985)
PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005; PMCID=PMC1075706
Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.
Exogenous ACE2 expression allows refractory cell lines to supps replication.
J. Virol. 79:3846-3850(2005)
PubMed=16494729; DOI=10.3201/eid1201.050496; PMCID=PMC3291385
Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)
PubMed=26235236; DOI=10.1016/j.jviromet.2015.07.016
Otto P.H., Reetz J., Eichhorn W., Herbst W., Elschner M.C.
Isolation and propagation of the animal rotaviruses in MA-104 cells -- 30 years of practical experience.
J. Virol. Methods 223:88-95(2015)
PubMed=28046048; DOI=10.1371/journal.pone.0169391; PMCID=PMC5207746
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)
Cross
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
再熬夜就真「傻」了！新研究发现，熬夜会降低大脑「排毒」效率，增加痴呆风险……
式的 HSPGs，并添加荧光标记的 Aβ42。结果显示无论肝素酶浓度如何，添加肝素酶 I、II 和 III 都会消除 Aβ42 吞噬作用的振荡。这证实了 HSPGs 水平的昼夜振荡，节律性地抑制了巨噬细胞清除 Aβ42 的作用。 图片来源：PLOS Genetics HSPGs 与 Aβ 肽结合抑制巨噬细胞对 Aβ42 的清除作用 小鼠 Aβ42（mAβ42）与人 Aβ42 几乎相同，只有三个氨基酸位点（R5G、Y10F 和 H13R）的区别。然而，这些差异都存在于已知的肝素结合区域内，影响
，此时只可记为一个细胞。如果团块很多，则可能需要重新吹打甚至消化直至绝大多数细胞为单个细胞血细胞计数器计数原则计算细胞数：血细胞计数器的机构示意图 由血细胞计数器的构造可以看出，血细胞计数器每个大方块可以容纳的体积为： 1 mm2×0.1 mm = 0.1 mm3 = 10-4 cm3 = 10-4 ml也即每个大方块的体积为万分之一毫升，因此在计算每毫升液体中的细胞数时需要乘以104。在常规没有使用台盼兰染色时，可以以下面公式计算每毫升培养基中细胞的个数：每毫升培养基中细胞的个数 = 每个方块内细胞
- - - - - - - - - 1mM Trolox - - - - - - - - - 1μM 一、hPSC 培养 1、将 hPSC 接种在 Vitronectin 包被的 6 孔板上，加入 hPSC 培养基进行扩增培养。 2、传代时加入 Accutase，将 hPSC 消化成单细胞。 3、用 DPBS 清洗两次后，接种到用 Vitronectin 包被的 12 孔板中，接种密度为 1.0 × 104 个细胞/cm2。 4、在传代培养的第一天加入含 10
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