SH-SY5Y细胞

SH-SY5Y/SH-SY5Y细胞系/SH-SY5Y细胞株/SH-SY5Y人神经母细胞瘤细胞

Cell line name SH-SY5Y

Synonyms SH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental

Accession CVCL_0019

Resource Identification Initiative To cite this cell line use: SH-SY5Y (RRID:CVCL_0019)

Comments Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: ENCODE project common cell types; tier 3.

From: Memorial Sloan Kettering Cancer Center; New York; USA.

Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810.

Population: Caucasian.

Characteristics: Neuroblastic type (N-type) (PubMed=15720811).

Characteristics: There seem to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096).

Virology: Low susceptibility to infection by Zika virus (ZIKV) (PubMed=29468137).

Doubling time: >55 hours (DSMZ=ACC-209).

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep tyrosine phosphoproteome analysis.

Omics: GPI-anchored proteins analysis by proteomics.

Omics: Mitochondrial proteome analysis by 2D-DE/MS.

Omics: N-glycan profiling.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Misspelling: SH-SY5; Note=Occasionally.

Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.

PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2

Akudugu J.M., Slabbert J.P., Serafin A.M., Bohm L.

Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.

Radiat. Res. 153:62-67(2000)

 

PubMed=11668190; DOI=10.1177/002215540104901105

Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.

Immunocytochemical analysis of cell lines derived from solid tumors.

J. Histochem. Cytochem. 49:1369-1378(2001)

 

PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1

Scheffler N.K., Miller S.W., Carroll A.K., Anderson C.M., Davis R.E., Ghosh S.S., Gibson B.W.

Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.

Mitochondrion 1:161-179(2001)

 

PubMed=12505268; DOI=10.1016/S0165-4608(02)00523-x

Spengler B.A., Biedler J.L., Ross R.A.

A corrected karyotype for the SH-SY5Y human neuroblastoma cell line.

Cancer Genet. Cytogenet. 138:177-178(2002)

 

PubMed=14676279; DOI=10.1242/jcs.00834

Goldschneider D., Blanc E., Raguenez G., Barrois M., Legrand A., Le Roux G., Haddada H., Benard J., Douc-Rasy S.

Differential response of p53 target genes to p73 overexpression in SH-SY5Y neuroblastoma cell line.

J. Cell Sci. 117:293-301(2004)

 

PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809

Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.

Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.

Cancer Res. 64:3395-3405(2004)

 

PubMed=15720811; DOI=10.1593/neo.04310; PMCID=PMC1531688

Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.

Characteristics of stem cells from human neuroblastoma cell lines and in tumors.

Neoplasia 6:838-845(2004)

 

PubMed=16822308; DOI=10.1186/1471-2407-6-177; PMCID=PMC1533846

Dam V., Morgan B.T., Mazanek P., Hogarty M.D.

Mutations in PIK3CA are infrequent in neuroblastoma.

BMC Cancer 6:177.1-177.10(2006)

 

PubMed=17506115; DOI=10.1002/nbm.1181

Peet A.C., McConville C.M., Wilson M.P., Levine B.A., Reed M., Dyer S.A., Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.

1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.

NMR Biomed. 20:692-700(2007)

 

PubMed=18957096; DOI=10.1186/1756-0500-1-95; PMCID=PMC2615028

Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.

Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.

BMC Res. Notes 1:95.1-95.11(2008)

 

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004; PMCID=PMC2913027

Holzel M., Huang S.-D., Koster J., Ora I., Lakeman A., Caron H.N., Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C., Messiaen L.M., Versteeg R., Bernards R.

NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.

Cell 142:218-229(2010)