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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
DKK-1/Dickkopf-Related Protein 1
- 供应商：
武汉恩玑生命科技有限公司
- 规格：
10ug/50ug/500ug
| 规格： | 10ug | 产品价格： | ￥380.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥1180.0 |
| 规格： | 500ug | 产品价格： | ￥9200.0 |
| 产品名称 |
Recombinant Human DKK-1 (C-Fc) |
| 英文名称 |
DKK-1/Dickkopf-Related Protein 1 |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a human IgG1 Fc tag at the C-terminus. |
| Accession |
O94907 |
| 表达宿主 |
Human Cells |
| 种属 |
Human |
| 预测分子量 |
52.7 KDa |
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 运输方式 |
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 分子别名 |
| Dickkopf-related protein 1; Dickkopf-1; Dkk-1 |
| 背景介绍 |
| Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Dkk-1(Dkk-1)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Dkk-1 单抗包被于酶标板上，标准品和样品中的 Dkk-1 与单抗结合，加入生物素化的抗大鼠 Dkk-1 ，形成免疫复合物连接
Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC
Recombinant monoclonal antibodies (rMAbs) are becoming major human therapeutics to treat life-threatening diseases such as cancer. These rMAbs are produced using either in vitro cell culture processes or transgenic technology in animals
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