- 询价
- 百欧博伟
- bio-110712
- 国内
- 2026年04月17日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
E. coli MP13
- 保质期：
3年
- 供应商：
百欧博伟
- 保存条件：
-80
平台编号：bio-110712
产品名称： 人 MMP13 (NM_002427) cDNA克隆
Accession： NM_002427
基因别名： CLG3; MANDP1
基因描述： Homo sapiens matrix metallopeptidase 13 (collagenase 3) (MMP13), mRNA.
CDS区长度： 1416
CDS区参考序列： 点击查看序列
翻译后氨基酸长度： 471
翻译后氨基酸参数序列：
点击查看序列
基因简介： Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology
规格： 10ul 质粒
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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
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