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- 2025年09月08日
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- 英文名：
HiCrome E. coli Agar
- 供应商：
上海西宝
- 规格：
100G
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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
Transformation of E. coli by Electroporation
antibiotic SOC medium 实验步骤 1. Inoculate a single colony of E. coli from a fresh agar plate into a flask containing 50 ml of LB medium. Incubate the culture overnight at 37°C with vigorous aeration (250 rpm in a rotary
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
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