BTK抗体;BTK Polyclonal Antibody

Description Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. References 1. Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240. 2. Thomas, J.D., et al. 1993. Science. 261: 355-358. 3. Tamagnone, L., et al. Oncogene 9: 3683-3688. Specification Aliases:BTK Entrez GeneID:695 Swissprot:Q06187 clone:7F12H4 WB Predicted band size:77kDa Host/Isotype:Mouse IgG1 Antibody Type:Primary antibody Storage:Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity:Human,Monkey Immunogen:Purified recombinant fragment of BTK expressed in E. Coli. Formulation:Ascitic fluid containing 0.03% sodium azide. Application WB:1/500 - 1/2000 IHC:1/200 - 1/1000 ICC:1/200 - 1/1000 ELISA:1/10000