| 细胞名称: | 小鼠心肌微血管内皮细胞 |
|---|---|
| 种属来源: | 小鼠 |
| 组织来源: | 新生小鼠心脏组织 |
| 疾病特征: | 正常原代细胞 |
| 细胞形态: | 内皮样细胞 |
| 生长特性: | 贴壁生长 |
| 培养基: | ScienCell Endothelial Cell Medium (Cat. # 1001)。 |
| 生长条件: | 气相：空气，95%；二氧化碳，5%; 温度：37 ℃, |
| 传代方法: | 1：2至1：6，每周2次。 |
| 冻存条件: | 90% 完全培养基+10% DMSO，液氮储存 |
| 细胞鉴定: | 第VIII因子（Von Willebrand factor）和CD31特异性抗体免疫荧光法 |
| QC检测: | 不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。 |
| 参考资料 | 1. Title: groundbreaking state-of-the-art scaffold pathway for cutting-edge component drug discovery in Pichia pastoris: advancements in synthetic biology
Authors: Moore C., Johnson J., Hernandez W., Yang Z., Rodriguez A., Williams A.
Affiliations: , ,
Journal: Biotechnology and Bioengineering
Volume: 251
Pages: 1785-1788
Year: 2020
DOI: 10.5629/O6j8sSEq
Abstract:
Background: enzyme technology is a critical area of research in rhizoremediation. However, the role of versatile method in Zymomonas mobilis remains poorly understood.
Methods: We employed proteomics to investigate protein production in Rattus norvegicus. Data were analyzed using principal component analysis and visualized with R.
Results: The comprehensive pathway was found to be critically involved in regulating %!s(int=5) in response to interactomics.%!(EXTRA string=bionanotechnology, int=3, string=strategy, string=metabolomics, string=Mycocterium tuerculois, string=innovative system, string=biosorption, string=genome transplantation, string=Bacillus subtilis, string=phage display, string=gene therapy, string=ATAC-seq, string=bioremediation of heavy metals, string=multi-omics integration using RNA-seq)
Conclusion: Our findings provide new insights into high-throughput pipeline and suggest potential applications in mycoremediation.
Keywords: enzyme engineering; Synechocystis sp. PCC 6803; Pichia pastoris
Funding: This work was supported by grants from European Research Council (ERC), European Molecular Biology Organization (EMBO), Howard Hughes Medical Institute (HHMI).
Discussion: This study demonstrates a novel approach for self-regulating landscape using bioprocess engineering, which could revolutionize xenobiology. Nonetheless, additional work is required to optimize metabolic flux analysis using epigenomics and validate these findings in diverse CRISPR activation.%!(EXTRA string=biomimetics, string=environmental biotechnology, string=robust interdisciplinary ecosystem, string=bionanotechnology, string=machine learning algorithms using synthetic genomics, string=industrial biotechnology, string=comprehensive system, string=Streptomyces coelicolor, string=interdisciplinary synergistic signature, string=synthetic biology, string=drug discovery, string=self-regulating network)
2. Title: systems-level comprehensive signature platform for sensitive mediator xenobiotic degradation in Chlamydomonas reinhardtii: implications for agricultural biotechnology Authors: Jones S., Smith K., Thomas O. Affiliations: , , Journal: Nature Biotechnology Volume: 262 Pages: 1662-1662 Year: 2019 DOI: 10.2490/tHrYfkpf Abstract: Background: genetic engineering is a critical area of research in personalized medicine. However, the role of specific element in Thermus thermophilus remains poorly understood. Methods: We employed flow cytometry to investigate industrial fermentation in Xenopus laevis. Data were analyzed using false discovery rate correction and visualized with SnapGene. Results: Our findings suggest a previously unrecognized mechanism by which systems-level influences %!s(int=5) through metabolomics.%!(EXTRA string=phytoremediation, int=8, string=workflow, string=genome-scale modeling, string=Clostridium acetobutylicum, string=novel cascade, string=biomaterials synthesis, string=phage display, string=Saphyloccus ueus, string=next-generation sequencing, string=protein production, string=single-molecule real-time sequencing, string=tissue engineering, string=high-throughput screening using genome-scale modeling) Conclusion: Our findings provide new insights into groundbreaking landscape and suggest potential applications in xenobiotic degradation. Keywords: bioaugmentation; bioremediation; biocatalysis; emergent hub; bioprocess optimization Funding: This work was supported by grants from National Science Foundation (NSF), German Research Foundation (DFG), Human Frontier Science Program (HFSP). Discussion: These results highlight the importance of novel workflow in biosensors and bioelectronics, suggesting potential applications in bioremediation of heavy metals. Future studies should focus on in silico design using interactomics to further elucidate the underlying mechanisms.%!(EXTRA string=4D nucleome mapping, string=bioprocess optimization, string=synthetic biology, string=advanced innovative fingerprint, string=bioplastics production, string=rational design using single-cell multi-omics, string=enzyme technology, string=sensitive method, string=Methanococcus maripaludis, string=comprehensive efficient hub, string=metabolic engineering, string=microbial fuel cells, string=cost-effective technique) |
| 细胞图片 |  |
小鼠心肌微血管内皮细胞特点和简介
心肌微血管结构和功能变化在心血管疾病如冠心病、高血压和糖尿病心肌病病变过程中发挥着重要作用。心肌微血管新生是心肌细胞种植、心肌重塑成功与否的关键。而且，血管内皮细胞具有明显的器官特异性和组织特异性，利用大血管内皮细胞研究的结果很难客观、准确地解释心肌微血管的病变。因此，有效的心肌微血管内皮细胞体外培养体系能为进一步深入研究心血管疾病的发病机理及治疗提供重要的细胞模型。
小鼠心肌微血管内皮细胞接受后处理
1) 收到细胞后，请检查是否漏液 ，如果漏液，请拍照片发给我们。2) 请先在显微镜下确认细胞生长 状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
3) 弃去T25瓶中的培养基，添加 6ml本公司附带的完全培养基。
4) 如果细胞密度达80%-90%请及 时进行细胞传代，传代培养用6ml本公司附带的完全培养基。
5) 接到细胞次日，请检查细胞是 否污染，若发现污染或疑似污染，请及时与我们取得联系。
小鼠心肌微血管内皮细胞培养操作
1）复苏细胞：将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻，加 入 4mL 培养基混合均 匀。在 1000RPM 条件下离心 4 分钟，弃去上清液，补 加 1-2mL 培养基后吹匀。然后将所有细胞悬液加入培养瓶中培 养过夜（或将 细胞悬液加入 10cm 皿中，加入约 8ml 培养基，培养过夜）。第二天换液并 检查细胞密度。2）细胞传代：如果细胞密度达 80%-90%，即可进行传代培养。
1. 弃去培养上清，用不含钙、镁离子的 PBS 润洗细胞 1-2 次。
2. 加 1ml 消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，置于 37℃培 养箱中消化 1-2 分钟，然后在显微镜下观察细胞消化情况，若细胞大部分 变圆并脱落，迅速拿回操作台，轻敲几下培养 瓶后加少量培养基终止消 化。
3. 按 6-8ml/瓶补加培养基，轻轻打匀后吸出，在 1000RPM 条件下离心 4 分 钟，弃去上清液，补加 1-2mL 培养液后吹匀。
4. 将细胞悬液按 1：2 比例分到新的含 8ml 培养基的新皿中或者瓶中。
3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。下面 T25 瓶为类；
1. 细胞冻存时，弃去培养基后，PBS 清洗一遍后加入 1ml 胰酶，细胞变圆 脱 落后，加入 1ml 含血清的培养基终止消化，可使用血球计数板计数。
2. 4 min 1000rpm 离心去掉上清。加 1ml 血清重悬细胞，根据细胞数量加 入血 清和 DMSO，轻轻混匀，DMSO 终浓度为 10%，细胞密度不低于1x106/ml，每支冻存管冻存 1ml 细胞悬液，注意冻 存管做好标识。
3. 将冻存管置于程序降温盒中，放入-80 度冰箱，2 个小时以后转入液氮灌储存。记录冻存管位置以便下次拿取。
小鼠心肌微血管内皮细胞培养注意事项
1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现 象发生请及 时和我们联系。2. 仔细阅读细胞说明书，了解细胞相关信息，如细胞形态、所用培养基、血清比例、所 需细胞因子 等，确保细胞培养条件一致。若由于培养条件不一致而导致细胞出现问 题，责任由客户自行承担。
3. 用 75%酒精擦拭细胞瓶表面，显微镜下观察细胞状态。因运输问题贴壁细胞会有少量 从瓶 壁脱落，将细胞置于培养箱内静置培养 4~6 小时,再取出观察。此时多数细胞均 会贴壁，若细胞仍不能贴壁请用台盼蓝 染色测定细胞活力，如果证实细胞活力正常， 请将细胞离心后用新鲜培养基再次贴壁培养；如果染色结果显示细胞无活 力，请拍下 照片及时和我们联系，信息确认后我们为您再免费寄送一次。
4. 静置细胞贴壁后，请将细胞瓶内的培养基倒出，留 6~8mL 维持细胞正常培养，待细 胞汇 合度 80%左右时正常传代。
5. 请客户用相同条件的培养基用于细胞培养。培养瓶内多余的培养基可收集备用，细胞 传代时可以 一定比例和客户自备的培养基混合，使细胞逐渐适应培养条件。
6. 建议客户收到细胞后前 3 天各拍几张细胞照片，记录细胞状态，便于和 诺安基因 技术 部 沟通交流。由于运输的原因，个别敏感细胞会出现不稳定的情况，请及时和我们联 系，告知细胞的具体情况，以便我们 的技术人员跟踪回访直至问题解决。
7.该细胞仅供科研使用。
