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- TD-0003M3
- 2025年11月24日
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- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 级别：
科研级
- 规格：
25 μl/100 μl/200 μl
BSA mouse monoclonal antibody （12C3-HRP）
| Product Name | BSA mouse monoclonal antibody （12C3-HRP） |
| Cat.# | TD-0003M3 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 50% glycerol, pH 7.0-7.4 |
| Product Imformation | |
| Description | BSA mouse monoclonal antibody |
| Conjugate | HRP |
| Applications | ELISA 1:2000-5000 |
| Reactivity | Bovine |
| Host species | Mouse |
| Purification | Protein A/G Affinity Purification |
| Form | Liquid |
| Concentration | |
| Purity | >95% by SDS-PAGE |
| Accession | P02769 |
| Alternative Names | Albumin，BSA, ALB |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4℃ short term (1-2 weeks).Store at -20℃ 12 months.Store at -80℃ long term. |
| Note | For research use only. |
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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