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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 保存条件:
-20
- 规格:
100ul
PRODUCT SPECIFICATIONS
Product name: FITC-Rabbit Anti- Duck IgY(IgG)(H+L)
Catalog No.: SAB20701F
Physical State: Liquid
Suggested Dilution Range:
Flow-Cyt/IF=1:250-1:2500
Storage Buffer: PBS, pH 7.4.
Storage Instructions: Store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: The antibody reacts with whole molecule rabbit IgG. The antibody may cross-react with immunoglobulins from other species.
Note: This product is for research use only,not for diagnostic or therapeutic use.
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
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