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Leishmania donovani杜氏利什曼虫(黑热病病原虫)染料法荧光定量PCR试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验利什曼病(Visceral Leishmaniasis)，是由杜氏利什曼原虫引起、经白蛉传播的慢性地方性传染病。临床上以长期不规则发热、进行性脾肿、消瘦、 贫血 、白细胞减少及血浆球蛋白增高为特征。 黑热病人，示肝脾肿大 （L. donovani - Hepatosplenomegaly ）From T. Evans 黑热病人，示脾肿大
佚名 杜氏利什曼原虫引起人与动物的杜氏利什曼原虫病，又称黑热病。在印度，患者皮肤上常有暗的色素沉着，并有发热，故又称Kala-azar，即黑热的意思。 一、人是怎么得黑热病的？ 黑热病（Kala--azar）又称内脏利什曼病(Visceral Leishmaniasis)，是由杜氏利什曼原虫引起、经白蛉传播
寄生在人体网状内皮细胞中，能引起黑热病，故又称黑热病原虫。我国长江以北有此病流行。此虫最早于1900年发现于印度。利什曼原虫的生活史有两个阶段，一段寄生于人或狗体内，另一段寄生于一种昆虫——白蛉体内，故黑热病是靠白蛉吸血而传播的。利什曼原虫在人体的巨噬细胞内，呈近圆形小体，只有2～3微米长，无鞭毛。可在细胞内进行二分裂生殖，破坏巨噬细胞，导致肝脾肿大，发烧，贫血，死亡率很高。当白蛉吸病人血时，利什曼原虫即进入白蛉的消化道内，逐渐变为梭形小体，长15～20微米，前端具一鞭毛
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