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Potato Virus M(PVM)马铃薯病毒M型RT-PCR试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验在拉丁文中是“毒”（ virus）的意思，以后转意成“病原体”。当莱弗勒（ F． Loeffler）和弗罗施（ P． Frosch）于 1898年发现通过细菌滤器的口蹄疫滤液具有感染能力时，就把它称为“超显微滤过病毒”（ ultramicroscopic filterable viruses）。 1899年杰林克（ M． W． Beijerinck）把烟草花叶病的病原体通过细菌滤器时也发现其滤液有感染能力，因而他称它为“传染性活汁”（ contagium
Meristem-Tip Culture for Propagation and Virus Elimination
variation are minimized. Fig. 1. A freshly excised meristem tip from an axillary bud of the potato Sotanum tuberosum . The two smallest emergent leaf primordia are present. Scale bar represents 50 μ M .
为流行性腮腺炎的病原，属于副粘病毒（ para－ myxovirus）科副粘病毒属。是 RNA型病毒。病毒粒子直径为 90— 600毫微米，球状。感染后出现腮腺炎、胰腺炎，有时也可出现脑膜炎。青春期以后患本病时发生睾丸炎和卵巢炎的比率较高，有时成为不妊症的原因。可使各种动物的红细胞凝集，并有溶血和细胞融合能力。可感染一些猴（弥猴、豚尾猴、 M． maura等）。接种到孵化 7天的鸡胚尿腔内能很好地增殖。使用 Hela细胞、猴肾细胞株能够传代（并能形成持续感染系）。
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