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- 详细信息
- 文献和实验
- 技术资料
一、服务流程:
接受订单及样品→RNA探针标记→与胞浆蛋白提取液孵育,形成RNA-蛋白质复合物→与磁珠结合,洗脱→WB检测或者质谱鉴定→结果交付
二、客户提供:
1、RNA序列或者RNA序列构建的质粒(种属);
2、相同种属的细胞样品。
三、最终交付:
1、基因合成测序报告;
2、构建含目的基因的质粒、菌液,测序报告;
3、体外转录模版的sense&antisense引物、模版电泳图;
4、RNA Pull-down蛋白SDS-PAGE银染;
5、质谱鉴定报告。
四、服务说明:
| 服务项目 | 细分 | 服务周期 |
| RNA pull dow | 未知蛋白(质谱鉴定) | 8周 |

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文献和实验Genome‐Wide Location Analysis by Pull Down of In Vivo Biotinylated Transcription Factors
provides a detailed protocol for genome?wide location analysis of in vivo biotinylated transcription factors by streptavidin pull?down followed by high?throughput sequencing (bioChIP?seq). Curr. Protoc. Mol. Biol. 92:21.20.1?21.20.15. © 2010 by John Wiley
, however, it is fine to use regular DNA-style TBE agarose gels. In either case, the RNA should initially be denatured (steps 2-3) and RNAse free reagents should be used. Procedure Add 2 µL RNA (up to 20 µg) to 18 µL 1x Reaction Buffer.
Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling jars with Eppendorf tubes, and weighing chemicals to prepare solutions. 2. Pull weighing paper or weighing boat from middle of pile. Tap chemicals
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