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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant A_s_p_ergillus fumigatus Protein ecm33 (ecm33)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥27960.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6654.0 |
| 规格: | 20μg | 产品价格: | ¥2376.0 |
纯度:
Greater than 95% as determined by SDS-PAGE.基因名:
ecm33Uniprot No.:
Q4WNS8别名:
/种属:
A_s_p_ergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
65.9 kDa表达区域:
20-372aa氨基酸序列:
ANCGKTDETITISSQSDADGYSSCSTIKGTIEIDEHLSGAITFNNVKQIDGTLSCTGGANISSIAAPMLNSIADTFKLDGLTTLTTLSFPSLTSVGSIQWTALPQLQSLDFTKGVNEAGDVTITNTGLANLNGISLNTVGKFDITENTQLKSININNLKNATGLINFAGNLNSLEVELPNLSSGTNMTFRNVSAVSVPSLHNLTGQLGFWGDTFKSFSAPNLTETGDLVFNSNSKLTNISMPALETVNGGFLITRNDELSSIDLPSLKVVTGAVDFSGKFDEVSMPKLENVKGQFNLQSTGNFSCDTFDKAHNDKVIRGTYKCKAAEPNPTTKDG_S_SGTTTSSG_S_SASASKSN蛋白标签:
C-terminal hFc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Involved in conidial and mycelial cell wall biogenesis.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官
C下265 x g离心5分钟。 吸出上清液并用 4 mL 1X PBS 洗涤一次。 4 °C下265 x g离心5分钟并吸出上清液。 轻弹锥形管的底部,使沉淀物脱落。 将 ECM基质胶置于 TC 操作台中,快速取出 1 mL 并将其添加到步骤 16 中脱落的细胞沉淀中。 用设置为 900 µL 的 P-1000 移液管在 ECM 基质胶中轻轻重悬细胞沉淀。 避免在在悬浮过程中引入气泡。 立即将细胞悬液置于冰上 5 分钟以防止凝胶化。 以1:50-1:100 将 1 mL ECM基质
4902). 成骨分化培养基: 向α-MEM (M4526) 培养基中添加10% FBS (ES-020-B), 1x 谷氨酰胺 (TMS-002), 1x P/S 双抗 (P4333), 50 μg/mL L-抗坏血酸 (A4403), 10 mM β-磷酸甘油 (G9422) and 100 ng/mL 骨形态发生蛋白2 (BMP-2) (SRP3326). Add 200 µl/well 人类脂肪MSC 细胞悬于3D扩增培养基中,以30,000 cells/well的细胞
h,同上收集培养上清液 用双抗体夹心ELISA 方法测HSC-T6培养上清液中PⅢP ,CⅣ的含量。以上每组均设3个复孔,具体操作严格按照试剂盒说明进行。 1.3 统计学方法 采用SPSS12.0统计软件,进行Spearman相关分析,单因素ANOVA(方差分析),两样本比较采用LSD-t检验,实验数据均以(x±s)表示,P<0.05判定有显著性差异。 2 结果 2.1 HMGB1对 HSC-T6分泌PⅢP、CⅣ的剂量效应 HMGB1能促进
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