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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dactylis glomerata Pollen allergen Dac g 3, partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dactylis glomerata Pollen allergen Dac g 3, partial品名简称:
Recombinant Dactylis glomerata Pollen allergen Dac g 3 protein, partial货号:
CSB-YP309006DAC规格:
20μg价格:
2616LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P93124别名:
Allergen Dac g III Allergen: Dac g 3种属:
Dactylis glomerata (Orchard grass) (Cock's-foot grass)蛋白长度:
Partial来源:
Yeast分子量:
12.9 kDa表达区域:
1-96aa氨基酸序列:
VKVTFKVEKGSDPKKLVLDIKYTRPGDTLAEVELRQHGSEEWEPLTKKGNLWEVKSSKPLTGPFNFRFMSKGGMRNVFDEVIPTAFKIGTTYTPEE蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Cloning, sequencing and immunological characterization of Dac g 3, a major allergen from Dactylis glomerata pollen."Guerin-Marchand C., Senechal H., Bouin A.P., Leduc-Brodard V., Taudou G., Weyer A., Peltre G., David B.Mol. Immunol. 33:797-806(1996)研究领域:
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
, α-MF, active483Pisum sativum copper amine oxidaseS, 1.4 mg/L, active338Pisum sativum mitochondrial pyruvate dehydrogenaseI, active343Plantago lanceolata pollen allergen Pla l 1S, 20 mg/L, active489Pokeweed antiviral proteinS, 12 mg/L, α-MF, active348
) 。 ( 3 ) 384 孔微量滴定板(Gentix,Christchurch,UK ) 。 ( 4 ) 96 孔深孔板:每孔容积 2 ml 的 96 孔微滴定板(Qiagen,Hilden,Germany) 。 ( 5 ) 96 针复制器(Nunc,Wiesbaden,Germany) 。 ( 6 ) 4 X SB 培养基:48 g/L 胰化蛋白胨,96 g/L 酵母提取物,0.8% ( V/V)甘油,120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液(磷酸钾盐缓冲
with glycosidases. Anal. Biochem.149, 218-224. 21. Faye, L., G o m o r d , V., Fitchette-Laine, A.-C., and Chrispeels, M . J. (1993) Affinity purification of antibodies specific for Asn-linked glycans containing al->3 fucose or (3l->2 xylose. Anal. Biochem
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