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- 瑞宁Rainin
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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州威佳科技有限公司
- 保修期：
广州一级代理
- 规格：
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文献和实验在RT-PCR中避免DNA污染（Avoiding DNA Contamination in R
was isolated according to protocol by five different methods. 0.5 µg RNA was used in RT-PCR reactions with Ambion's RETROscript® Kit. PCR reactions were performed with 5 µg RNA. 10 µl of each reaction was electrophoresed on a 2% agarose gel and stained
of each dNTP, 250 nM of each primer and 1/10 volume of a 10x PCR standard buffer (15 mM MgCl2; 100 mM Tris/HCl, pH 8.3; 500 mM KCl) are added to a total volume of 50 µl. The PCR is run in the thermal cycler GeneAmp 9600 (Perkin Elmer). The PCR products
C. elegans whole mount immunohistochemistry with Bouin's Fixative
in my lab). The supernatant, save 50 µl, is removed by aspiration. A mix of 400 µl of Bouin's, 400 µl methanol and 10 µl of ßME is placed in the glass tube to initiate fixation and subsequently the worms are transferred to an eppendorf tube using a glass
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