IG0010 G-418 遗传霉素硫酸盐 抗感染 索莱宝

CAS：108321-42-2

分子量：692.71

纯度：≥98%

靶点：Bacterial

 

 

 

使用本产品的应用案例（仅供参考）

In Vitro

Cell（HepG2.2.15 cells，380 µg/mL G-418 sulfate）

Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM;Solarbio) and supplemented with 10% fetal bovine serum (FBS).G-418 sulfate (380 µg/mL;Solarbio) was added to DMEMwith 10% FBS to maintain HepG2.2.15 cells.All cells were incubated at 37℃ with 5% CO2.

来源文献：Guo C, Ouyang Y, Cai J, Xiong L, Chen Y, Zeng X, Liu A. High expression of IL-4R enhances proliferation and invasion of hepatocellular carcinoma cells. Int J Biol Markers. 2017 Oct 31;32(4):e384-e390. doi: 10.5301/ijbm.5000280. PMID: 28665449.

 

Cell（（HepG2.2.15 cells，300 µg/mL G-418）

HepG2.2.15 cells were incubated in minimum Eagle’s medium containing 10% FBSand 300 µg/mL G418 (Solarbio, Beijing, China).

来源文献：Jiang W, Wang L, Zhang Y, Li H. Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/TNFAIP3 Axis. Cancer Manag Res. 2020 Nov 2;12:11031-11040. doi: 10.2147/CMAR.S272983. PMID: 33173336;PMCID: PMC7648158.

 

Cell（OC cells，800 μg/mL  G418）

PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively,by G418 (Solarbio, Shanghai, China)at a concentration  of 800 μg/mL and puromycin (Solarbio, Shanghai, China)at a concentration of 2 μg/mL.

来源文献：Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978;PMCID: PMC7348179.

 

Cell（Replicon cell，500 μg/ml G418）

Replicon cell lines were selectedand maintainedin 500 μg/ml G418 (Geneticin;Solarbio, China).

来源文献：Sobhanimonfared F, Bamdad T, Roohvand F. Cross talk between alcohol-induced oxidative stress and HCV replication. Arch Microbiol. 2020 Sep;202(7):1889-1898. doi: 10.1007/s00203-020-01909-9. Epub 2020 May 24. PMID: 32448963.

 

Cell（A549, H446 and 95-D cells,500 µg/mL G418）

To generate P65 overexpression cell lines, the full length of p65 cDNA was inserted into pCMV-Flag vector and transfected intoA549, H446 and 95-D cells, then selected with 500 µg/mL G418 (Solarbio, IG0010) to generate p65 stable expression cell lines.

来源文献：Zeng C, Zou T, Qu J, Chen X, Zhang S, Lin Z. Cyclovirobuxine D Induced-Mitophagy through the p65/BNIP3/LC3 Axis Potentiates Its Apoptosis-Inducing Effects in Lung Cancer Cells. Int J Mol Sci. 2021 May 29;22(11):5820. doi: 10.3390/ijms22115820. PMID: 34072333;PMCID: PMC8199090.