Rabphilin 3A (phospho-Ser237)

v468 chapter 5 糖原合成酶激酶-3 的抑制

of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt

C3裂解产物（C3SP）C3a的定量检测

C3是补体二条激活途径的关键分子，C3活化后裂解为C3a、C3b两个片段，C3a很快被血清羧肽酶N裂解为C3adesarg，C3b被H、I因子灭活为C3bi，经蛋白酶水解为C3c、C3d、C3dg等，检测这些裂解产物即可反映C3的活化程度。C3a的定量检测采用竞争性抑制放射免疫法。其原理为用“I标记C3（I-C3）”与待测样本、兔抗人C3a多克隆抗体一起孵育，待测样本中的C3a（未标记抗原）能竞争性地与抗C3a结合，使C3a复合物（B）增多，而“I-C3”游离出来（F），根据B%来判断待测样本

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –