Rabphilin 3A (phospho-Ser237)

别名:KIAA0985; Rabphilin-3A; Exophilin-1
计算分子量:76KD
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting:1:500 - 1:1000
Immunohistochemistry:1:50 - 1:100
Immunofluorescence:1:100 - 1:300
图片:

Western blot analysis of Rabphilin 3A (phospho-Ser237) expression in Raw264.7 Calyculin treated whole cell lysates.

,

Immunohistochemical analysis of Rabphilin 3A (phospho-Ser237) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

,

Immunofluorescent analysis of Rabphilin 3A (phospho-hospho-Ser237) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.