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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
杭州联科生物技术股份有限公司
- 规格:
125ml
1.产品简介
流式染色缓冲液适用于悬浮细胞的免疫荧光染色实验,为含BSA的缓冲液。本缓冲液可用于抗体和细胞的稀释,以及表面染色和流式分析的洗涤阶段。本缓冲液所含的动物血清蛋白可减少抗体的非特异性结合。
2.产品特点
▪即用型,使用方便
▪可降低抗体非特异性结合
▪价格便宜,常备现货
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文献和实验A general protocol for staining cell for cytometry analysis
2 µl PI and incubate for 15 min at RT. 5.Add 400 µl of 1× Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr). Note: Methods for utilizing Annexin V binding on adherent cells ( i.e., monolayer
Intracellular Immunofluorescent Staining for Flow Cytometry
. No. 00-8222 ) eBioscience Permeabilization Buffer (Cat. No. 00-8333 ) eBioscience Flow Cytometry Staining Buffer (Cat. No. 00-4222 ) Instruments Pipettes and pipettors Centrifuge Ice bucket or refrigerator Flow
Intracellular Cytokine Staining for Analysis by Flow Cytometry
To determine the function of a particular cell type, it is necessary either to have a large number of similar (ideally identical) cells or to use extremely sensitive methods to detect the activity of a single cell. Lymphocytes present special
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