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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 英文名:
GDNF/ATF/HSCR3,Human,Recombinant
- 规格:
10μg/50ug/500ug/1mg
| 规格: | 10μg | 产品价格: | ¥1780.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥4980.0 |
| 规格: | 500ug | 产品价格: | ¥17480.0 |
| 规格: | 1mg | 产品价格: | ¥25680.0 |
产品信息
| 别名 | ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI with polyhistidine tag at the C-terminus. |
| 检索号 | P39905.1 |
| 分子量 | 16.01 kDa |
| 表观分子量 | 16 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is <10 ng/mL. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍

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-ChIP and X-ChIP. - http://www.abcam.com/index.html?pageconfig=resource&rid=10418&CFID=2422773&CFTOKEN=36329956 Expert protocols and tips for N-ChIP and X-ChIP. AbCAM Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http
(9): 1245–53. doi:10.1101/gr.6406307. PMID 17690206. ^ Bertone P, Stolc V, Royce TE, Rozowsky JS, Urban AE, Zhu X, Rinn JL, Tongprasit W, Samanta M, Weissman S, Gerstein M, Snyder M (December 2004). "Global identification of human transcribed sequences
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