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FGF-2/ bFGF/ FGF-b (K128N), Hu

man, Recombinant
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  • ¥980 - 11180
  • Procell
  • PCK005
  • 武汉
  • 2025年07月01日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      武汉益普生物科技有限公司

    • 英文名

      FGF-2/ bFGF/ FGF-b (K128N), Human, Recombinant

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥980.0
    规格:50ug产品价格:¥1880.0
    规格:500ug产品价格:¥7880.0
    规格:1mg产品价格:¥11180.0

    产品信息

    别名Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
    物种Human
    表达宿主E.coli
    序列信息Met1-Ser155
    检索号BAG70135.1
    分子量17.2kDa
    表观分子量17kDa

     

    产品特性

    纯度>95% as determined by reducing SDS-PAGE.
    内毒素<1.0 EU/µg as determined by LAL test.
    保存Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C,stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
    运输The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
    制剂Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.

    背景介绍

    Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.

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