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产品中文名称：α-葡萄糖-ω-琥珀酰亚胺碳酸酯基聚乙二醇
产品英文名称：α-Glucose-ω-succinimidyl poly(ethylene glycol)
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文献和实验COMPLEX MEDIA YPAD medium or Also Called YPD plus Adenine Yeast Extract - Peptone - Dextrose plus Adenine medium 6.0 gm yeast extract (Difco) 12.0 gm Peptone (Difco) 12.0 gm Glucose 60 mg adenine hemisulphate 600
Yeast Transformation Using Frozen Competent Cells and Single-stranded DNA as a Carrier
2-3 more doublings.) Note: The cells must be grown in glycerol if you are going to freeze them. Glucose-grown cells lose competence upon freezing. 2. Pellet cells gently (~800 x g). Resuspend in 7-8 ml of 1X TE-LiAc . 3. Pellet cells gently. Resuspend in 1 ml
Methods for use with the mTn-3xHA/GFP-mutagenized library
. To derepress the GAL promoter, inoculate transformants into 2 mls SC-ura-leu with 2% raffinose as carbon source and grow to saturation. Dilute 1/100 into SC-leu with 2% galactose as carbon source (control: SC-leu with 2% glucose as carbon
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