CD3E & CD3G Heterodimer重组蛋白|Re

CD3E & CD3G Heterodimer重组蛋白|Recombinant Human CD3E & CD3G Heterodimer Protein(Fc - FLAG & Fc - His Tag)（产品说明）
蛋白名称：Human CD3E & CD3G Heterodimer Protein(Fc - FLAG & Fc - His Tag)
蛋白构建：A DNA sequence encoding the (Met1-Asp126) of human CD3E (NP_000724.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; A DNA sequence encoding the (Met1-Ser116) of human CD3G (NP_000064.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized Anti-CD3 antibody, mouse IgG2a at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3E & CD3G Heterodimer Protein(Fc - FLAG & Fc - His Tag) (Cat: CT123-H2303H), the EC50 is 50-150 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Asp 23 & Gln 23
蛋白分子量：The recombinant Human CD3E & CD3G consists of 694 (353+341) amino acids and has a calculated molecular mass of 78.36 (39.77+38.59) KDa.As a result of glycosylation, the apparent molecular mass of mouse CD3E & CD3G heterodimer is approximately 47.10 and 42.93 KDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000724.1 & NP_000064.1
蛋白氨基酸序列：Met1-Asp126 & Met1-Ser116
蛋白标签：C-human IgG1-Fc & Flag & C-human IgG1-Fc & His