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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from internal of Human SLC25A11.
- 亚型:
/
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
/
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q02978
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB;WB:1:500-1:3000
- 浓度:
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点:
SLC25A11
- 抗体英文名:
SLC25A11 Antibody
- 抗体名:
SLC25A11;SLC20A4;Mitochondrial 2-oxoglutarate/malate carrier protein;OGCP;Solute carrier family 25 member 11
- 规格:
100μl
保存缓冲液
/功能
Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism.Iacobazzi V., DNA Seq. 3:79-88(1992).
Yu W., Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Colinge J., Submitted (OCT-2008) to UniProtKB.
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文献和实验我现在做elisa,目的是利用此法来检测我样品中蛋白的浓度,我用的是改良的双抗体夹心法,就是包被鼠单抗,加标准品和待测样品,再加兔多抗,再加山羊抗兔的酶标二抗,TMB显色。我现在的问题是摸索单抗和多抗的稀释度,所以就将多抗稀释了三个浓度1:5000,1:10000,1:15000,1:20000,单抗 包被稀释度是1:15000,标准品都是五倍稀释即:25μg/mL , 5μg/mL , 1μg/mL , 0.2μg/mL , 0.04μg/mL 酶标二抗是1:5000 双波长A450
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secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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