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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around site of Tri-methyl-Lys (27) derived from Human Histone H3.1
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P68431
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, IF; Recommended dilution: IF:1:50-1:200
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
HIST1H3A
- 抗体英文名:
Tri-methyl-HIST1H3A (K27) Antibody
- 抗体名:
Histone H3/l antibody
- 规格:
100μl/50μl/20μl
| 规格: | 100μl | 产品价格: | ¥2420.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥1320.0 |
| 规格: | 20μl | 产品价格: | ¥660.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验法所替代。利用微球菌核酸酶的切割特性(在染色质核小体之间的 DNA 更容易被切开),可以快速温和地将染色质 DNA 切断,且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态,使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒,含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体(Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620)、阴性对照抗体、DNA
). CoBATCH for high-throughput single-cell epigenomic profiling. bioRxiv, 590661. [6] Carter, B., Ku, W. L., Tang, Q., Kang, J. Y., & Zhao, K. (2019). Mapping Histone Modifications in Low Cell Number and Single Cells Using Antibody-guided Chromatin
Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
背景就比较必要了)。相关链接:如何判断 ChIP 实验是否成功?Active Motif ChIP 实验相关产品:ChIP 抗体ChIP 试剂盒磁珠/琼脂糖珠其他参考文献:1. Santos-Rosa, H., et al., Active genes are tri-methylated at K4 of histone H3. Nature, 2002. 419(6905): p. 407-11.2. Bernstein, B.E., et al., Genomic maps
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