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- 英文名：
Fructanase Mixture (Ultrapure, recombinant, liquid)
- 保质期：
一年
- 保存条件：
-20℃
- 规格：
10 mL / 20,000 Units
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For Fructan Determination.
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.
Components: exo-Inulinase (2,000 U/mL on kestose, at 40oC) and endo-Inulinase (100 U/mL on inulin at 40oC).
Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
| Content: | 10 mL / 20,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In 50% (v/v) glycerol plus 0.02% sodium azide |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Fructanase |
| EC Number: | endo-Inulinase: 3.2.1.7, exo-Inulinase: 3.2.1.80 |
| CAZy Family: | GH32 |
| CAS Number: | 9001-57-4, 9025-67-6, 37288-56-5 |
| Synonyms: | endo-inulinase: 1-beta-D-fructan fructanohydrolase exo-inulinase: fructan β-fructosidase |
| Source: | Asper-gillus niger |
| Concentration: | endo-inulinase: Supplied at ~ 1,000 U exo-inulinase: Supplied at ~ 10,000 U |
| Expression: | Recombinant from Asper-gillus niger |
| Specificity: | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
| Unit Definition: | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC. |
| Temperature Optima: | 60oC |
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