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- 详细信息
- 文献和实验
- 技术资料
- 库存:
39
- 英文名:
NO-Indomethacin
- CAS号:
301838-28-8
- 供应商:
上海莼试
- 保存条件:
Store at -20°C
- 规格:
1mg 5mg 10mg 50mg
分子式:C28H30ClN3O9S
分子量:620.1
溶解度:DMF: 30 mg/ml,DMF:PBS (pH 7.2)(1:1): .5 mg/ml,DMSO: 20 mg/ml,Ethanol: .5 mg/ml
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
NO-indomethacin is a hybrid molecule of indomethacin and a nitric oxide (NO) donor. This drug design combines the anti-inflammatory properties of a non-steroidal anti-inflammatory drug (NSAID) with the gastrointestinal protective effects of NO. Compounds of this class retain their anti-inflammatory and analgesic activity, but have reduced gastrointestinal and kidney toxicity compared to the NSAID alone. NO-indomethacin also enhances the cancer chemopreventative activity of indomethacin. NO-indomethacin exhibits an IC50 of 82 μM, compared to >1,000 μM for indomethacin alone, for the inhibition of pancreatic cancer cell (PaCa-2) growth after 24 hours in culture.
商品属性:
| 货号 | CS-01Y76570 | 规格 | 1mg 5mg 10mg 50mg |
| CAS号 | 301838-28-8 | 分子量 | 620.1 |
| 含量 | >98.00% | 别名 | N/A |
| 分子式 | C28H30ClN3O9S | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
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1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
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文献和实验Quantitation of Indomethacin in Serum and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
Indomethacin is a non-narcotic and non-steroidal anti-inflammatory drug used in the treatment of various inflammatory diseases such as rheumatoid arthritis, osteoarthritis, and ankylosing spondylitis. In neonates, it is also used
Nonprimate Animal Models of H. pylori Infection
would colonize. Mongolian gerbils particularly after gastric lesions were produced by indomethacin (4 ); however, this work has never been substantiated nor followed up. Instead, most attention was paid subsequently to the use of naturally occurring Helicobacter
Isolation and Differentiation of Amnion-Derived Stem Cells (ADSCs)
indomethacin. After 3 days in the AIM, ADSCs are cultured in adipogenic maintenance media (AMM) for 3 days. AMM consists of 10% FBS/DMEM and 10 μg/ml insulin in a base of DMEM. ADSCs are maintained at 37°C for 3 weeks, with media changed
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