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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rickettsia rickettsii Chaperonin GroEL (groEL), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rickettsia rickettsii Chaperonin GroEL (groEL), partial品名简称:
Recombinant Rickettsia rickettsii groEL protein, partial货号:
CSB-EP422667RNC规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
groELUniprot No.:
A8GT30别名:
(60 kDa chaperonin)(Chaperonin-60)(Cpn60)种属:
Rickettsia rickettsii (strain Sheila Smith)蛋白长度:
Partial来源:
E.coli分子量:
25.3 kDa表达区域:
319-483aa氨基酸序列:
TAKRVTISKENTVIVDGNGDKKNIEDRVLQIKSQIAETTSDYDKEKLQERLAKLSGGVAVLKVGGATEVEVKERKDRVEDALAATRAAVEEGVVAGGGVTLLHASQPLTKLKVENKDQQAGIEIVIEALKDPLKQIVENAGENGGVVVGKLLEHKDKNYGFNAQD蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.功能2:
/文献:
/研究领域:
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文献和实验CD60a/CD60b/CD60c 分子 CD60a 常用单克隆抗体或代号:GD3(R24) 主要表达细胞: Tsub,Pt,Bsub,Mac,M,C[CH0] 功 能: T细胞活化增殖;诱导凋亡过程中线粒体通透性的改变;恶性黑素瘤的标志 CD60b 常用单克隆抗体或代号:9-O-acety-GD3 (UM4D4,M-T6004) 主要表达细胞:Tsub,Ba,Mac,M,Ep[CH0] 功
装4个小试管,每管1ml;(2) 加2单位溶血素,每管lml;(3) 加2%绵羊红细胞,每管lml;(4) 37℃水浴30分钟;(5) 2000rpm离心5分钟;(6) 取上清液测光密度值(540nm波长)。表1 CH50测定的标准曲线制备试管号12345678910112%血红素(ml)——0.10.20.30.40.50.60.70.80.91.02%SRBC(ml)1.00.90.80.70.60.50.40.30.20.1——PBS(ml)2.02.02.02.02.02.02.02.02.02
等量的溶血素(2U/ml)。充分混合。置37℃水浴中作用30分钟,每隔10分钟摇动一次以免细胞下降即成5×108/ml致敏羊红细胞。 3、补体CH50单位测定 (1)按下表加样; (2)将各管充分混合,置37℃水浴中60分钟(摇动1~2次,以免红细胞下沉); (3)从水浴中取出后,立即将各管以2000转/分离心10分钟,将各管上清液分别移于另一试管; (4)测定各管上清液OD值(波长=540nm)。
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