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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rickettsia conorii Outer membrane protein B (ompB), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
ompBUniprot No.:
Q9KKA3别名:
(168 kDa surface-layer protein)(Cell surface antigen 5)(Sca5)(Surface protein antigen)(rOmp B)(rOmpB)(120 kDa outer membrane protein OmpB)(Surface protein antigen)(p120)种属:
Rickettsia conorii (strain ATCC VR-613 / Malish 7)蛋白长度:
Partial来源:
E.coli分子量:
43.8 kDa表达区域:
1335-1655aa氨基酸序列:
GALRYLGTPETAEMAGPEAGAIPAAVAAGDEAVDNVAYGIWAKPFYTDAHQSKKGGLAGYKAKTTGVVIGLDTLANDNLMIGAAIGITKTDIKHQDYKKGDKTDVNGFSFSLYGAQQLVKNFFAQGSAIFSLNQVKNKSQRYFFDANGNMSKQIAAGHYDNMTFGGNLTVGYDYNAMQGVLVTPMAGLSYLKSSDENYKETGTTVANKQVNSKFSDRTDLIVGAKVAGSTMNITDLAVYPEVHAFVVHKVTGRLSKTQSVLDGQVTPCISQPDRTAKTSYNLGLSASIRSDAKMEYGIGYDAQISSKYTAHQGTLKVRVNF蛋白标签:
C-terminal 6xHis-Avi-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
The 120 kDa surface-exposed protein is a major structural protein which may play a role as a rickettsial virulence factor and/or immunogen during infection. ; The 32 kDa beta peptide may serve as a membrane anchor. It has been shown to adhere to biotinylated Vero cell proteins.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
【Jounal club】开张大吉!蛋白质技术:一篇关于衣原体对我影响很深的文章。
文章标题:蛋白质技术:一篇关于衣原体对我影响很深的文章。 作者:Klein M, Kotz A, Bernardo K, Kronke M. 文题:Detection of Chlamydia pneumoniae-specific antibodies binding to the VD2 and VD3 regions of the major outer membrane protein. 杂志全名(或缩写):J Clin Microbiol. 年份,卷(期
bodies, Biopolymers (peptide science) 51 (1999) 297-307. 4. E.B. Clark, Refolding of recombinant proteins, Curr. Opin. Biotechnol. 9 (1998) 157-163. 5. E.B. Clark, Protein refolding for industrial processes, Curr. Opin. Biotechnol. 12 (2001) 202-207
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