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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Protein Wnt-3a (Wnt3a), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥12372.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2880.0 |
| 规格: | 20μg | 产品价格: | ¥1536.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Wnt3aUniprot No.:
P27467别名:
/种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
40.9 kDa表达区域:
130-250aa氨基酸序列:
EGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESR蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Developmental BiologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验protein, Drosophila Wnt8 (Dwnt8 or WntD), indicating that this protocol is likelyapplicable to all Wnts. Key words: Wnt , Wnt3A , b-catenin , Purification , Blue Sepharose , Immobilized metal affinitychromatography (IMAC) , Gel filtration , Heparin
三句话读懂一篇 CNS:百岁老人的长寿秘诀;雄性激素越高越有
物 ATPase7/CDC50C 在疟原虫按蚊感染和传播过程中的功能和分子机制,揭示了疟原虫躲避按蚊宿主免疫识别和清除的潜在新机制。疟原虫每年导致全球数十万人死伤,望其早日被消灭!图 3:来源 Science Advances4. Nature Communications: 解析蛋白 Wntless 与 Wnt3a 复合物结构Wnt 蛋白是一类蛋白质信号分子,Wnt 信号转导活动异常导致多种疾病。2021 年 7 月 27 日,西湖大学马丹与周强课题组在 Nature Communications 上发表
miaoduan 本人刚刚开始做实验,有关wnt通路的检测,想请教激活wnt通路的方法,看资料主要有三种: 1,加wnt蛋白 2,LiCl抑制gsk3β 3,转染wnt质粒或者点突变的β-catenin质粒 不知哪种方法可行,具体如何操作呢 非常感谢!!! edwardellen 加wnt3a,看过一篇CANCER CELL是这样激活的 liubodoctor
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