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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Protein Wnt-3a (Wnt3a), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Protein Wnt-3a (Wnt3a), partial品名简称:
Recombinant Mouse Wnt3a protein, partial货号:
CSB-EP026136MO1规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Wnt3aUniprot No.:
P27467别名:
/种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
40.9 kDa表达区域:
130-250aa氨基酸序列:
EGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESR蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).功能2:
/文献:
/研究领域:
Developmental Biology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验protein, Drosophila Wnt8 (Dwnt8 or WntD), indicating that this protocol is likelyapplicable to all Wnts. Key words: Wnt , Wnt3A , b-catenin , Purification , Blue Sepharose , Immobilized metal affinitychromatography (IMAC) , Gel filtration , Heparin
肠上皮是成年哺乳动物中自我更新最快的组织,平均自我更新时间少于 5 天。 肠干细胞位于肠隐窝(intestinal crypt)底部附近,每个隐窝中的干细胞大约有 4-6 个,它们产生快速增殖的转运扩增 (TA) 细胞,肠细胞、杯状细胞和肠内分泌细胞都从 TA 细胞发育而来。 TA 细胞快速分裂、转运扩增的子细胞占据了隐窝的其余部分,并流向绒毛的侧面,在那里它们分化、吸收营养,最终在绒毛尖端凋亡【1】。 在体外可以由 hPSCs 来模拟胚胎肠发育的过程,通过添加高浓度的 FGF4 和 Wnt3A
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
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