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人PDE5A重组蛋白cGMP-binding cGMP-sp

ecific phosphodiesterase;CGB-PDEHuman cGMP-specific 3',5'-cyclic phosphodiesterase(PDE5A)蛋白
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  • ¥1836
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  • CSB-YP524921HU
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) , partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Human cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) , partial

    品名简称:

    Recombinant Human PDE5A protein, partial

    货号:

    CSB-YP524921HU

    规格:

    20μg

    价格:

    1836

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    PDE5A

    Uniprot No.:

    O76074

    别名:

    cGMP-binding cGMP-specific phosphodiesterase;CGB-PDE

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Partial

    来源:

    Yeast

    分子量:

    41.0 kDa

    表达区域:

    534-875aa

    氨基酸序列:

    EEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN

    蛋白标签:

    C-terminal 6xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    25-35 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.

    功能2:

    /

    文献:

    /

    研究领域:

    Cancer

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    相关实验
    • Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP

      equilibrium-binding constant of 600 nM causing a total fluorescence intensity ratio change of 45%. In contrast, cAMP could elicit a maximal 10% change in fluorescence resonance energy transfer (FRET) ratio, demonstrating an approx 500-fold selectivity for cGMP

    • cGMP-Immunocytochemistry

      Immunocytochemistry (ICC) of small, water-soluble molecules requires some special precautions compared to ICC of large protein molecules. In general, protein antigens are relatively insensitive to the type of fixative used. Apparently

    • Hyperspectral Imaging of FRET-Based cGMP Probes

      :365–70, 2008). The probes are comprised of known cGMP binding sites—e.g., from phosphodiesterase type 5 (PDE5) or protein kinase G (PKG)—attached to fluorescent proteins. Binding of cGMP triggers conformational changes that alter the emitted

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    ¥1836