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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Hordeum vulgare Gamma-hordein-3
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Hordeum vulgare Gamma-hordein-3品名简称:
Recombinant Hordeum vulgare Gamma-hordein-3 protein货号:
CSB-EP304465HWQ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P80198别名:
/种属:
Hordeum vulgare (Barley)蛋白长度:
Full Length来源:
E.coli分子量:
40.6 kDa表达区域:
1-289aa氨基酸序列:
ITTTTMQFNPSGLELERPQQLFPQWQPLPQQPPFLQQEPEQPYPQQQPLPQQQPFPQQPQLPHQHQFPQQLPQQQFPQQMPLQPQQQFPQQMPLQPQQQPQFPQQKPFGQYQQPLTQQPYPQQQPLAQQQPSIEEQHQLNLCKEFLLQQCTLDEKVPLLQSVISFLRPHISQQNSCQLKRQQCCQQLANINEQSRCPAIQTIVHAIVMQQQVQQQVGHGFVQSQLQQLGQGMPIQLQQQPGQAFVLPQQQAQFKVVGSLVIQTLPMLCNVHVPPYCSPFGSMATGSGGQ蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Has a role in the transport and targeting of prolamins to the vacuole of developing barley endosperm.功能2:
/文献:
"A role for gamma 3 hordein in the transport and targeting of prolamin polypeptides to the vacuole of developing barley endosperm." Rechinger K.B., Simpson D.J., Svendsen I., Cameron-Mills V. Plant J. 4:841-853(1993)研究领域:
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文献和实验通过转基因改良谷物种子蛋白质成分的研究。例如,通过基因操作可以改变小麦中高分子质量(HMW) 的谷蛋白亚基的组成,而并不影响小麦农艺性状 [ 42 ] 。最近研究发现,高水平表达高分子质量亚基1D x 5 和/或 1Dy10 的转基因小麦,其面团具有较髙的搅拌强度和韧性 [ 43 ] 。一项相关研究表明,两份分别含有约 20 个和 50 个 HMW 谷蛋白单基因拷贝及其启动子的转基因小麦株系中,检测到了相关的染色质解凝过程[ 44 ] 。在反义抑制硫氧还蛋白积累的转基因株系中,已检测到谷蛋白的降解
P. G. (2002) Endospermspecific expression of green fluorescent protein driven by the hordein promoter is stably inherited in transgenic barley( Hordeum vulgare) plants. Physiol. Plant. 115, 144-154. 23. Datla, R. S. S
剂(1% Ignite Hoechst-Roussel Agri-Vet Company, NJ) ( 5 ) 蛋白提取缓冲液:50 mmol/L 磷酸钠缓冲液(pH 7.0 ) ,10 mmol/L EDTA(乙二胺四乙酸), 0.1% (V/V) Triton X-100,0.1% (m/V ) Sarkosyl,10 mmol/L 疏基乙醇,10 mmol/L PMSF (phenylmethylsulfonyl fluoride)。 ( 6 ) 碱性
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