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- 详细信息
- 文献和实验
- 技术资料
- 库存:
39
- 英文名:
TAE-1
- CAS号:
1414469-59-2
- 供应商:
上海莼试
- 保存条件:
Store at 2°C to 8°C
- 规格:
1mg 5mg 10mg 25mg
| 货号 | CS-01Y77173 | 规格 | 1mg 5mg 10mg 25mg |
| CAS号 | 1414469-59-2 | 分子量 | 1128.6 |
| 含量 | >98.00% | 别名 | |
| 分子式 | C39H51N6O9 • 3I | 化学名 | 2,2',2'-[1,3,5-triazine-2,4,6-triyltris(oxy-4,1-phenylenecarbonyloxy)]tris[N,N,N-trimethyl-ethanaminium]triiodide |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C39H51N6O9 • 3I
分子量:1128.6
溶解度:Acetonitrile: 5 mg/ml,DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: Partially soluble,PBS (pH 7.2): 5 mg/ml
储存条件:Store at 2°C to 8°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
IC50: 0.3 ± 0.02 μM for AChE; 3.9 ± 0.2 μM for BuChETAE-1 is an inhibitor of amyloid-β fibril formation and aggregation.Alzheimer’s disease (AD), a progressive neurodegenerative disorder, is characterized by the cerebral accumulation of insoluble aggregates of amyloid-β peptides (Aβ). Although the precise mechanisms governing neuronal loss remains ambiguous, toxicity resulting from Aβ-activated pathways is evident.In vitro: In a previous study, the authors examined the effects of TAE-1 on differentiated human SH-SY5Y neuronal cells grown in tissue culture. Results showed that the stimulation of neuronal cellular process length and branching was noted. Moreover, the increased synaptophysin suggested that TAE-1 could stimulate synapse formation. Increased expression of MAP2 was also orved, indicating that TAE-1 promoted the differentiation of human neurons. In addition, targeted AChE inhibition was evaluated by electrochemical quantification of the enzymatic product, thiocholine, on unmodified gold screen-printed electrodes. It was found that at increasing TAE-1concentrations, there was a corresponding decrease in the AChE activity resulting in reduced amount of oxidizable thiocholine. The IC50 value was found to be 0.465 μM for TAE-1 [1].In vivo: Up to now, there is no animla in vivo data reported.Clinical trial: So far, no clinical study has been conducted.
注意事项:
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
使用方法:
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
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文献和实验为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。 TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间
一般先配制50倍的TAE缓冲液,用时再稀释成1倍的。 50倍TAE缓冲液的配制方法为 1. 称取下列试剂于1L烧杯中: Tris 242g Na2 EDTA.2H2 O 37.2g 2.向烧杯中加入约600mL的去离子水,充分搅拌溶解
A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Species
Large insert libraries are valuable tools for the positional cloning of genes of interest, physical mapping of chromosomes, comparative genomics, and molecular breeding. There are five types of large DNA insert libraries: cosmid, yeast
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