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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥2480.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5880.0 |
MCP-1/CCL2重组蛋白|Recombinant Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag), Biotinylated(产品说明)
蛋白名称:Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag), Biotinylated
蛋白构建:A DNA sequence encoding the mouse CCL2 (NP_035463.1) (Met1-Asn148) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
表达宿主:HEK293 Cells
蛋白纯度:> 80 % as determined by SDS-PAGE
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 24
蛋白分子量:The mouse CCL2 consists of 135 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_035463.1
蛋白氨基酸序列:Met1-Asn148
蛋白标签:C-His
蛋白名称:Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag), Biotinylated
蛋白构建:A DNA sequence encoding the mouse CCL2 (NP_035463.1) (Met1-Asn148) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
表达宿主:HEK293 Cells
蛋白纯度:> 80 % as determined by SDS-PAGE
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 24
蛋白分子量:The mouse CCL2 consists of 135 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_035463.1
蛋白氨基酸序列:Met1-Asn148
蛋白标签:C-His
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文献和实验相关实验
。当某一个标签的使用,一是能构成表位利于纯化和检测;二是构成独特的结构特征(结合配体)利于纯化。组氨酸残基侧链与固态的镍有强烈的吸引力,可用于固定化金属螯合层析(IMAC),对重组蛋白进行分离纯化。使用His-tag有下面优点:1.标签的分子量小,只有~0.84KD,而GST和蛋白A分别为~26KD和~30KD,一般不影响目标蛋白的功能;2.His标签融合蛋白可以在非离子型表面活性剂存在的条件下或变性条件下纯化,前者在纯化疏水性强的蛋白得到应用,后者在纯化包涵体蛋白时特别有用,用高浓度的变性剂溶解
技术资料暂无技术资料 索取技术资料
Sino Biological MCP-1/CCL2重组蛋白 小鼠 His标签 生物素化 mcp1重组蛋白
¥2480 - 5880








