- ￥1790 - 3220
- Sino Biological
- 北京
- 50013-M08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse Carbonic Anhydrase XIV / Car14 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10 µg/20 µg/50 µg
| 规格： | 10 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 20 µg | 产品价格： | ￥3220.0 |
| 规格： | 50 µg | 产品价格： | ￥3220.0 |
Carbonic Anhydrase XIV/CA14重组蛋白|Recombinant Mouse Carbonic Anhydrase XIV / Car14 Protein (His Tag)（产品说明）
蛋白名称：Mouse Carbonic Anhydrase XIV / Car14 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 16
蛋白分子量：The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035927.1
蛋白氨基酸序列：Met1-Met290
蛋白标签：C-His
蛋白名称：Mouse Carbonic Anhydrase XIV / Car14 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 16
蛋白分子量：The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035927.1
蛋白氨基酸序列：Met1-Met290
蛋白标签：C-His
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文献和实验相关实验
包涵体 复性 二硫键 到目前为止，人们表达的重组蛋白质已有4000多种，其中用E.coli表达的蛋白质要占90%以上，尽管基因重组技术为大规模生产目标蛋白质提供了崭新的途径，然而人们在分离纯化时却遇到了意想不到的困难，即这些蛋白质在E.coli中绝大多数是以包涵体形式存在，重组蛋白不仅不能分泌到细胞外，反而在细胞内聚集成没有生物活性的直径约0.1~3.0μm的固体颗粒[1]。自从应用大肠杆菌体系表达基因工程产品以来，人们就一直期望得到高活性、高产量的重组蛋白。不可溶、无生物活性的包涵体必须
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Sino Biological Mouse CA14重组蛋白(His Tag) 高纯度 高活性
￥1790 - 3220
