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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
at-20℃
- 库存：
99
- 宿主：
Rabbit
- 应用范围：
ELISA
- 浓度：
见说明
- 抗体英文名：
Anti-FMC63 scFv Monoclonal antibody (SAA1996)
- 规格：
50-100ug
Rabbit Anti-FMC63 scFv单克隆抗体(克隆号SAA1996)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
Rabbit Anti-FMC63 scFv单克隆抗体(克隆号SAA1996)更多优势产品欢迎新老客户选购。
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MAB1597 小鼠抗ASFV I177L单克隆抗体(克隆号1A013)
MAB1598 小鼠抗CSFV NS5B单克隆抗体(克隆号1A014)
MAB1599 小鼠抗CSFV NS5B单克隆抗体(克隆号1A015)
MAB1600 小鼠抗ASFV I10L单克隆抗体(克隆号1A016)
MAB1601 小鼠抗ASFV NS4A单克隆抗体(克隆号1A017)
MAB1602 小鼠抗ASFV NS4A单克隆抗体(克隆号1A018)
MAB1603 小鼠抗ASFV F778R单克隆抗体(克隆号1A019)
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文献和实验Generation of Rabbit Immune Libraries
for the combinatorial cloning of the variable heavy and light chain immunoglobulin repertoire of rabbits for the subsequent phage display of rabbit scFv antibody fragments is described.
1. 抗原及酶标记抗体 ①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No.A0423); ②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No.P0260);均为 DAKO 公司产品，使用时按照说明书要求稀释。 4. 洗涤液(含0.05%Tween 20的PBS)：1LPBS 中加入Tween-20 500μL。 5. 封闭液(含1%牛血
丰富，可构建多种类型的噬菌体免疫库和天然库（scFv、Fab、VHH等）。 2. 筛选：普健自有噬菌体天然抗体文库，序列正确性高，来源丰富，库容大。 3. 筛选通量高，已成功交付多例建库及筛选项目，客户分布于欧洲及亚洲。 4. 载体带有His标签，可直接用于表达抗体，方便抗体纯化验证。 5. 现已成功开发出兔scFv天然噬菌体文库产品：ATA-Rabbit Naïve ScFv I（库容：1.09×1010 pfu）。 经兔scFv天然库筛选出的序列，拼接成全长IgG重组
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