- 询价
- KA&M BIO
- MAB4960
- 2025年10月11日
- ELISA
- Human
- Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969) (DENV-2)
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 形态：
Liquid
- 保存条件：
at-20℃
- 适应物种：
Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969) (DENV-2)
- 库存：
99
- 宿主：
Human
- 应用范围：
ELISA
- 浓度：
见说明
- 抗体英文名：
Anti-DENV-2 Envelope protein E/EDII fusion loop Monoclonal antibody (E60#)
- 规格：
50-100ug
抗DENV-2 Envelope protein E/EDII fusion loop单克隆抗体(克隆号E60#)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
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文献和实验A chemokine-driven positive feedback loop organizes lymphoid follicles
, establishing a positive feedback loop that is likely to be important in follicle development and homeostasis. In germinal centres the feedback loop is overridden, with B-cell lymphotoxin 1 2 expression being induced by a mechanism
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
and e.g. 20% of bound fractions to SDS-PAGE, transfer to a nitrocellulose or PVDF membrane Detect GFP-fusion protein with an α-GFP antibody (e.g. from Roche, Mannheim, Germany) and interacting proteins with the respective antibodies
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
, and analysis. A protocol is described for enhanced detection of low?affinity interactions by generating multivalent complexes using Fc?fusion bait proteins and protein A microbeads, along with a statistical method for hit scoring and identification
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