- 询价
- KA&M BIO
- MAB4953
- 2025年10月11日
- ELISA
- Human
- Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969) (DENV-2)
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- 亚型：
IgG1, kappa
- 形态：
Liquid
- 保存条件：
at-20℃
- 适应物种：
Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969) (DENV-2)
- 库存：
99
- 宿主：
Human
- 应用范围：
ELISA
- 浓度：
见说明
- 抗体英文名：
Anti-DENV-2 Envelope protein E/EDIII A strand Monoclonal antibody (DV87.1)
- 规格：
50-100ug
抗DENV-2 Envelope protein E/EDIII A strand单克隆抗体(克隆号DV87.1)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
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文献和实验挑选14个克隆进行筛选，结果发现14个克隆均能与靶抗原有反应，其中以4（E4F4）、5（E5F5）、6（G4）、7（G5）、11（G9）克隆颜色较深，挑取6号克隆，利用APAAP法进一步检测其结合活性。结果显示，克隆6的表达产物能和Daudi细胞特异结合。克隆6的测序结果和氨基酸序列分析表明该基因序列完全符合PDB库中抗体所具有的若干保守的框架氨基酸的特点，该序列是一抗体基因序列。2、抗CD20嵌合抗体Fab和F（ab）2的表达与纯化SDS-PAGE电泳结果表明Protein G可有效纯化Fab
microarrays: performing multiplex immunoassays on a single chip. A n a l.C h em . 75, 4368-4372. 25. Kersten, B., Wanker, E. E., Hoheisel, J. D., and Angenendt, P. (2005) Multiplexing approaches in protein microarray technology. E x p e r t R ev . P r
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