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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
at-20℃
- 库存：
99
- 抗体英文名：
Anti-Human COL2A1 Monoclonal antibody (UL1)
- 规格：
50-100ug
小鼠抗人COL2A1单克隆抗体(克隆号UL1)上海圻明生物优势供应欢迎咨询。配对抗体小课堂：
Paired antibodies are two antibodies that can bind to an antigenic molecule at the same time. Specifically:
Paired antibodies are commonly used in sandwich ELISA experiments
An antigenic molecule usually has multiple epitopes, and different antibodies can target different epitopes
When two antibodies are able to bind to different epitopes of the same antigenic molecule at the same time, the two antibodies are called paired antibodies
However, not all antibodies against different epitopes can be paired antibodies. Sometimes, when an antibody binds to an antigen, it may cause the configuration of other binding sites to change, making it impossible for other antibodies to bind properly
Steric hindrance effects may also result in two antibodies with close binding sites not being able to bind to the antigen at the same time
Therefore, the preparation of paired antibodies usually requires two steps: first the antibody is prepared, and then the paired antibody is screened
Screening of paired antibodies is typically performed using a sandwich ELISA method of bispecific antibodies, in which different combinations of antibodies are tested to determine which antibodies can be successfully paired
Paired antibodies have important applications in immunology research and diagnostics, especially in assays that require high specificity and sensitivity.
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文献和实验SQ Blood Mini Protocol for 50ul Clotted Blood
2. Nuclease-free 1.5 ml microcentrifuge tubes 3. Water Bath preset at 37°C 实验步骤 1. Transfer the 50ul blood include any liquid residual into a 1.5 ml centrifuge tube. 2. Add 550ul WTL Buffer and pipet
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
1. Prepare a Lysis Mixture by mixing one volume of Buffer MRB and one volume of isopropanol. For 96 samples, combine15 ml Buffer MRB with 15 ml isopropanol and mix throughly by vortexing. Add 300 ul Lysis Mixture for each sample at step 3. 2. Add 50 ul
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