- ￥3870 - 8940
- Sino Biological
- 北京
- 11077-H41H-B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CEACAM5 Protein (Fc & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
CEACAM-5/CD66e重组蛋白|Recombinant Human CEACAM5 Protein (Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human CEACAM5 Protein (Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CEACAM5 (NP_004354.2)(Met1-Ala685) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Lys 35
蛋白分子量：The recombinant Human CEACAM5 consists of 904 amino acids and predicts a molecular mass of 99.87 kDa. It migrates as an approximately 136.68-203.96 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_004354.2
蛋白氨基酸序列：Met1-Ala685
蛋白标签：C-human IgG1-Fc & AVI
蛋白名称：Human CEACAM5 Protein (Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CEACAM5 (NP_004354.2)(Met1-Ala685) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Lys 35
蛋白分子量：The recombinant Human CEACAM5 consists of 904 amino acids and predicts a molecular mass of 99.87 kDa. It migrates as an approximately 136.68-203.96 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_004354.2
蛋白氨基酸序列：Met1-Ala685
蛋白标签：C-human IgG1-Fc & AVI
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文献和实验相关实验
连接就需要应用较强的增强组织通透性的试剂。增强组织通透性常用的方法如应用稀释的酸洗涤、去垢剂（detergent）或称清洗剂Triton X-100、酒精或某些消化酶如胃蛋白酶、胰蛋白酶、胶原蛋白酶和淀粉酶（diastase）等。这种广泛的去蛋白作用无疑可增强组织的通透性和核酸探针的穿透性，提高杂交信号，但同时也会减低RNA的保存最和影响组织结构的形态，因此，在用量及孵育时间上应慎为掌握。 蛋白酶K（Proteinase K）的消化作用在ISHH中是应用于蛋白消化的关键步骤，其浓度及孵育
技术资料暂无技术资料 索取技术资料
Sino Biological CEACAM5/CD66e重组蛋白 Biotinylated Fc&AVI标签 ceacam5
￥3870 - 8940
