- ￥3870 - 8940
- Sino Biological
- 北京
- 10699-H49H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD86 Protein (His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
B7-2/CD86重组蛋白|Recombinant Human CD86 Protein (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human CD86 Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CD86 (NP_008820.4) (Met1-His239) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE.
蛋白活性：Immobilized Recombinant Human CTLA4 / CD152 Protein (His Tag) (Cat:11159-H08H) at 2 μg/mL (100 μl/well) can bind Recombinant Human CD86 Protein (His & AVI Tag), Biotinylated (Cat:10699-H49H-B), the EC50 is 210-600 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Leu 20
蛋白分子量：The recombinant Human CD86 consists of 246 amino acids and predicts a molecular mass of 28.42 kDa. It migrates as an approximately 44.64-67.56 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_008820.4
蛋白氨基酸序列：Met1-His239
蛋白标签：C-His-AVI
蛋白名称：Human CD86 Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CD86 (NP_008820.4) (Met1-His239) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE.
蛋白活性：Immobilized Recombinant Human CTLA4 / CD152 Protein (His Tag) (Cat:11159-H08H) at 2 μg/mL (100 μl/well) can bind Recombinant Human CD86 Protein (His & AVI Tag), Biotinylated (Cat:10699-H49H-B), the EC50 is 210-600 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Leu 20
蛋白分子量：The recombinant Human CD86 consists of 246 amino acids and predicts a molecular mass of 28.42 kDa. It migrates as an approximately 44.64-67.56 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_008820.4
蛋白氨基酸序列：Met1-His239
蛋白标签：C-His-AVI
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文献和实验相关实验
共刺激作用。八十年代末至九十年代初期共刺激分子B7在肿瘤免疫研究中较为深入和广泛。 共刺激分子B7为44-54kD的糖蛋白，属于免疫球蛋白超家族成员。最初发现于活化的B细胞上，它也表达于树突状细胞、活化的巨噬细胞上。除B细胞来源的肿瘤外，其他肿瘤很少表达B7。共刺激分子的缺乏也是肿瘤的弱免疫原性的重要因素。B7又分为B7-1（又名为CD80）和B7-2（又名为CD86），两者仅有25%氨基酸同源。APC活化时，B7-2表达早于 B7-1。B7有两种受体存在于T细胞表面，CD28低亲和力受体
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Sino Biological CD86重组蛋白 生物素化 His&AVI标签 cd86 (b7-2)
￥3870 - 8940
