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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human ROR1 Protein (Fc & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg
| 规格: | 20 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥8940.0 |
蛋白名称:Human ROR1 Protein (Fc & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human ROR1 (NP_005003.2)(Met1-Glu403) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Anti-ROR1 Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human ROR1 Protein (Fc & AVI Tag), Biotinylated (Cat: 13968-H41H-B), the EC50 is 1.6-4.8 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 30
蛋白分子量:The recombinant Human ROR1 consists of 627 amino acids and predicts a molecular mass of 70.53 kDa. It migrates as an approximately 85.43 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_005003.2
蛋白氨基酸序列:Met1-Glu403
蛋白标签:C-human IgG1-Fc & AVI
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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
., Vukicevic, S., and Luyten, F.P. (1996) Primary structure and tissue distribution of Frzb, a novel protein related to Drosophila frizzled, suggest a role in human skeletal morphogenesis. J. Biol. Chem . 271 , 26131–26137. 6. Finch, P.W., He., X., Kelley, M
连接就需要应用较强的增强组织通透性的试剂。增强组织通透性常用的方法如应用稀释的酸洗涤、去垢剂(detergent)或称清洗剂Triton X-100、酒精或某些消化酶如胃蛋白酶、胰蛋白酶、胶原蛋白酶和淀粉酶(diastase)等。这种广泛的去蛋白作用无疑可增强组织的通透性和核酸探针的穿透性,提高杂交信号,但同时也会减低RNA的保存最和影响组织结构的形态,因此,在用量及孵育时间上应慎为掌握。 蛋白酶K(Proteinase K)的消化作用在ISHH中是应用于蛋白消化的关键步骤,其浓度及孵育
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