N/A蛋白|潘迪努斯威胁器N/A蛋白|潘迪努斯威胁器N/A重

Research Areas

Others

Uniprot ID

P59888

Gene Names

N/A

Alternative Name(s)

Imperatoxin I;IpTx1;IpTxi;Imperatoxin inhibitor;Imperatoxin I large subunit;Imperatoxin I small subunit

Lead Time

13-23 business days

Organism

Pandinus imperator (Emperor scorpion)

Source

E.coli

Expression Region

32-135aa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Target Protein Sequence

TMWGTKWCGSGNEATDISELGYWSNLDSCCRTHDHCDNIPSGQTKYGLTNEGKYTMMNCKCETAFEQCLRNVTGGMEGPAAGFVRKTYFDLYGNGCYNVQCPSQ

MW

18.4 kDa

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

/

Biological_Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance

Phospholipase toxin, which may catalyze the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Inhibits both skeletal (RYR1) and cardiac (RYR2) ryanodine receptors (calcium release channels). Probably blocks ryanodine receptors by generating a lipid product.

Function

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