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FOLR1/FOLR1蛋白Recombinant Human

Folate receptor alpha(FOLR1) (Active)重组蛋白FOLR蛋白
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  • ¥1368 - 22200
  • CUSABIO已认证
  • CN
  • CSB-MP008784HU1
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Folate receptor alpha(FOLR1), partial (Active)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥22200.0
    规格:100μg产品价格:¥2568.0
    规格:20μg产品价格:¥1368.0

    纯度:

    Greater than 95% as determined by SDS-PAGE.

    基因名:

    FOLR1

    Uniprot No.:

    P15328

    别名:

    FOLR

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Partial

    来源:

    Mammalian cell

    分子量:

    25.9 kDa

    表达区域:

    25-233aa

    氨基酸序列:

    RIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAAAM

    蛋白标签:

    C-terminal 10xHis-tagged

    产品提供形式:

    Lyophilized powder

    缓冲液:

    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells .

    内毒素:

    Less than 1.0 EU/ug as determined by LAL method.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    Active Protein & In Stock Protein

    Biological_Activity:

    Measured by its binding ability in a functional ELISA. Immobilized human FOLR1 at 2 μg/mL can bind Anti-FOLR1 recombinant antibody(CSB-RA008784MA1HU). The EC50 is 6.893-7.883 ng/mL.

    Research Areas:

    Others

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B. EMBL/GenBank/DDBJ databases (JUN-2004)

    Function:

    /

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    图标文献和实验
    该产品被引用文献
    Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B. EMBL/GenBank/DDBJ databases (JUN-2004)
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    • 蛋白复性(二)-基因重组蛋白质的体外复性

      X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白

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    图标技术资料

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    文献支持
    FOLR1/FOLR1蛋白Recombinant Human Folate receptor alpha(FOLR1) (Active)重组蛋白FOLR蛋白
    ¥1368 - 22200