万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
52
- 英文名:
Urocortin III (mouse) (trifluoroacetate salt)
- 供应商:
上海莼试
- 保存条件:
Store at -20°C
- 规格:
500μg
分子式:C186H312N52O52S2.XCF3COOH
分子量:4173
溶解度:DMSO: 1 mg/ml
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Urocortin III is a neuropeptide hormone and member of the corticotropin-releasing factor (CRF) family which includes mammalian CRF , urocortin , urocortin II , frog sauvagine, and piscine urotensin I.1 Mouse urocortin III shares 90, 40, 37, and 18% identity to human urocortin III , mouse urocortin II , human urocortin , and mouse urocortin, respectively. It is expressed in the hypothalamus, brainstem, lateral septum (LS)/bed nucleus of stria terminalis (BnST) in the CNS and the small intestine, pancreatic β-cells, and skin in the periphery in mice. Urocortin III selectively binds to type 2 CRF receptors (Kis = 5, 1.8, and >100 nM for rat CRF2α, rat CRF2β, and human CRF1, respectively). It stimulates cAMP production in CHO cells expressing rat CRF2α and mouse CRF2β (EC50s = 0.073 and 0.08 nM, respectively) as well as in cultured anterior pituitary cells expressing endogenous CRF2β. In vivo, urocortin III (2 μg, i.p.) accelerates metamorphosis of S. hammondii tadpoles.2 Urocortin III (20 μg, i.c.v.) increases exploration in the open field test and decreases latency to enter the lit compartment in the dark-light emergence test in mice, indicating anxiolytic-like activity, and mRNA expression is increased in mouse brain in response to restraint.3 It is co-released with insulin to potentiate glucose-stimulated somatostatin release in vitro and in vivo in wild-type mice, however, it is depleted from β-cells in mouse and macaque models of type 2 diabetes, suggesting a role in glycemic control.4
商品属性:
| 货号 | CS-01Y77407 | 规格 | 500μg |
| CAS号 | N/A | 分子量 | 4173 |
| 含量 | >95.00% | 别名 | N/A |
| 分子式 | C186H312N52O52S2.XCF3COOH | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
公司正在出售的产品:
| Boc-δ-azido-Nva-OH . DCHA | WZ4002 |
| WZ4003 | 干扰素ε检测试剂盒 IFNε ELISA Kit |
| WZ8040 | 干扰素γ诱导蛋白30检测试剂盒 IFI30 ELISA Kit |
| WZB117 | 干扰素α8检测试剂盒 IFNα8 ELISA Kit |
| X-376 | 干扰素γ诱导蛋白16检测试剂盒 IFI16 ELISA Kit |
| X77 | 干扰素α7检测试剂盒 IFNα7 ELISA Kit |
| Xaliproden hydrochloride | 干扰素α6检测试剂盒 IFNα6 ELISA Kit |
| Xanomeline (oxalate) | NF-H ELISA Kit |
| Xanthoangelol | 干扰素κ检测试剂盒 IFNκ ELISA Kit |
| Xanthopterin | 干扰素ω检测试剂盒 IFNω ELISA Kit |
| Xanthopterin (hydrate) | 干扰素调节因子1检测试剂盒 IRF1 ELISA Kit |
| Xanthoquinodin A1 | 干扰素调节因子4检测试剂盒 IRF4 ELISA Kit |
| Xanthorrhizol | Urocortin III (mouse) (trifluoroacetate salt)干扰素调节因子9检测试剂盒 IRF9 ELISA Kit |
| Hyocholic Acid | 高尔基磷蛋白3检测试剂盒 GOLPH3 ELISA Kit |
| 高尔基蛋白A2检测试剂盒 GOLGA2 ELISA Kit | 高尔基糖蛋白1检测试剂盒 GLG1 ELISA Kit |
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验% ),这使得化学合成siRNA 的成本升高。体外转录制备长片断RNA 并不难,但是由于研究表明长片断的双链RNA (>29bps )在哺乳动物细胞中通常会引发抗病毒反应―― 一种非特异基因表达抑制,而目前体外转录往往无法制备小于29bp 的小分子siRNA s 。一个新的研究发现,用RNase III 降解长片断双链RNA 成为siRNA s 库也能够有效诱导特异的基因沉默,这样可以避免了筛选有效siRNA s 的漫长过程,从而快速,经济的实现特定基因表达的沉默。在线虫、果蝇和其他一些物种的RNA 干扰
Inducing RNAi with siRNA Cocktails Generated by RNase III
products (21 bp range) can effectively mediate RNAi in both mammalian cells (10) and mouse embryos (11). We demonstrate here that the siRNA cocktails produced by complete digestion by RNase III (~12 to 15 bp) are capable of silencing specific genes
from their mothers ii. discard un-needed non-transgenic pups iii. house males and females separately f) when mice are 6 weeks old they may be mated (see 1. above) ES Cell-Mouse Chimera Breeding Based on our experience, we propose the following
技术资料暂无技术资料 索取技术资料









