IN-PP5E基因敲除质粒

pLenti-IN-PP5E-sgRNA (IN-PP5E基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的IN-PP5E基因敲除的质粒(L18220 pLenti-IN-PP5E-sgRNA)、慢病毒(L18221 IN-PP5E Knockout Lentivirus)、HEK293T细胞(L18222 IN-PP5E Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L18223 IN-PP5E Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L18224 IN-PP5E Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

IN-PP5E基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	IN-PP5E	56623	BC028032	NM_019892

 

About the gene
Official Symbol	IN-PP5E
Previous Symbol	JBTS1
Official Full Name	inositol polyphosphate-5-phosphatase E
Synonyms	PPI5PIV; CORS1; pharbin
Location	9q34.3
Gene Type	protein_coding
Uniprot ID	Q9NRR6
Pathway/Library	Phosphatases Library
Gene Summary	The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms.