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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
15-30℃
- 英文名:
Dextran(40,000)
- 供应商:
MP
- 规格:
0210150510(10g)/0210150550(50g)/0210150580(100g)
| 规格: | 0210150510(10g) | 产品价格: | ¥524.73 |
|---|---|---|---|
| 规格: | 0210150550(50g) | 产品价格: | ¥1123.1 |
| 规格: | 0210150580(100g) | 产品价格: | ¥1685.04 |
Dextran solutions have been used as plasma extenders. Dextran MW 40,000 has been used for the isolation of intact nuclei. Increase in threonine, serine, proline, and cysteine dietary supply can promote mucin synthesis in dextran sulfate sodium treated rats. Dextran 40 is used in the development of new improved preservation solutions for organ transplantation. Dextran 40 may be used to determine cell tightness and flux parameters across cell layers.Use of dextrans as long and hydrophilic spacer arms improves the performance of immobilized proteins acting on macromolecules. Dextrans are used in many applications as platelet aggregants, plasma volume extenders, osmotic pressure regulators, stabilizers, organ separation media, matrix components, copolymers, microcarriers, binding agents, viscosity modifiers, antithrombotics, lubricants and physical structure components. Dextrans may be used as long hydrophilic spacer arms to improve the performance (freedom of movement) of conjugated/bound proteins. Dextrans may be derivatized for use in biosensor systems.Dextran 40 may be derivatized for use as a cell permeation reagent.
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文献和实验至2~3cm高时,打开柱的出口,并注意控制操作压以均匀不变的流速直到胶装完为止。柱装好后,在床的上面盖上一张大小略小与柱内径的滤纸片,以防止样品中一些不溶物质混入床中和加样时凝胶被冲起。再以洗脱液平衡柱层,直至层析的胶床高不变为止。柱装得是否均匀,可用蓝色葡聚糖上柱检验;如果色带均匀下移,说明柱子已装好,可以使用。 三、上样 称取0.5mg蓝色葡聚糖2000(分子量200万以上)四份,分别放在称量瓶中,再称取标准蛋白牛血清白蛋白(Mr 67 000);卵清蛋白(Mr 43
实验原理 葡聚糖凝胶(Sephadex)过滤法测定蛋白质分子量的原理,主要是依据这种凝胶具有分子筛作用,一定型号的凝胶具有大体上一定大小的孔径。在一定的凝胶柱内,凝胶孔隙所占的体积称为内水容积Vi,凝胶颗粒间的自由空间所占的体积称为外水容积V0。当样品流经凝胶柱时,大于孔隙的大分子完全不滲入到凝胶内部,只需V0体积的洗脱液便可将其由一端洗脱到另一端;相反,如果样品体积小于孔隙,则需要V0+Vi体积的洗脱液,才能将它们由一端洗脱到另一
装完为止。柱装好后,在床的上面盖上一张大小略小与柱内径的滤纸片,以防止样品中一些不溶物质混入床中和加样时凝胶被冲起。再以洗脱液平衡柱层,直至层析的胶床高不变为止。柱装得是否均匀,可用蓝色葡聚糖上柱检验;如果色带均匀下移,说明柱子已装好,可以使用。 三、上样 称取0.5mg蓝色葡聚糖2000(分子量200万以上)四份,分别放在称量瓶中,再称取标准蛋白牛血清白蛋白(Mr 67 000);卵清蛋白(Mr 43 000);胰凝乳蛋白酶











