P2RY12基因敲除质粒

pLenti-P2RY12-sgRNA (P2RY12基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的P2RY12基因敲除的质粒(L29410 pLenti-P2RY12-sgRNA)、慢病毒(L29411 P2RY12 Knockout Lentivirus)、HEK293T细胞(L29412 P2RY12 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L29413 P2RY12 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L29414 P2RY12 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

P2RY12基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	P2RY12	64805	BC017898	NM_022788

 

About the gene
Official Symbol	P2RY12
Previous Symbol	-
Official Full Name	purinergic receptor P2Y12
Synonyms	P2Y12; SP1999; HORK3
Location	3q25.1
Gene Type	protein-coding gene
Uniprot ID	Q9H244
Pathway/Library	others
Gene Summary	The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelets aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene.