MAP4K2基因敲除质粒

pLenti-MAP4K2-sgRNA (MAP4K2基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的MAP4K2基因敲除的质粒(L28470 pLenti-MAP4K2-sgRNA)、慢病毒(L28471 MAP4K2 Knockout Lentivirus)、HEK293T细胞(L28472 MAP4K2 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L28473 MAP4K2 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L28474 MAP4K2 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

MAP4K2基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	MAP4K2	5871	BC047865	NM_004579

 

About the gene
Official Symbol	MAP4K2
Previous Symbol	RAB8IP
Official Full Name	mitogen-activated protein kinase kinase kinase kinase 2
Synonyms	GCK; BL44
Location	11q13.1
Gene Type	protein-coding gene
Uniprot ID	Q12851
Pathway/Library	others
Gene Summary	The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.