PPP1R15B基因敲除质粒

pLenti-PPP1R15B-sgRNA (PPP1R15B基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的PPP1R15B基因敲除的质粒(L18410 pLenti-PPP1R15B-sgRNA)、慢病毒(L18411 PPP1R15B Knockout Lentivirus)、HEK293T细胞(L18412 PPP1R15B Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L18413 PPP1R15B Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L18414 PPP1R15B Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

PPP1R15B基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	PPP1R15B	84919	BC065280	NM_032833

 

About the gene
Official Symbol	PPP1R15B
Previous Symbol	-
Official Full Name	protein phosphatase 1 regulatory subunit 15B
Synonyms	FLJ14744
Location	1q32.1
Gene Type	protein_coding
Uniprot ID	Q5SWA1
Pathway/Library	Phosphatases Library
Gene Summary	This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism.